Research
Research Interests
Sequencing Technology for Biological Dynamics
Our lab focuses on developing and optimizing novel high-throughput sequencing methods to study biological dynamics, including cell lineage tracing and RNA modifications (e.g., m6A, m5C, Ψ) at single-base resolution. We pioneer techniques like CAM-seq, PUM-seq, and RAD-seq to push the boundaries of epitranscriptomics and single-cell analysis.
Small RNA Targetome & RBP Footprinting
We develop and apply advanced sequencing technologies for RBP (RNA-Binding Protein) footprinting to map protein-RNA interactions across the transcriptome. Our research focuses on identifying small RNAs and non-coding RNAs, characterizing their targetomes, quantifying their abundance and proportions, and tracking their dynamic changes across different conditions. We investigate the diversity of RNA species, their biogenesis pathways, and how their targetome dynamics relate to biological processes.
RNA & Protein Design
Our work focuses on innovative RNA and protein design strategies, aiming to unravel and engineer complex biological functions. This includes integrating AI-driven approaches and developing novel tools for cell lineage tracing like SMALT to uncover how genetic and epigenetic networks evolve.
Evolutionary & Systems Biology
We investigate developmental and evolutionary history by integrating multi-omics data. We also explore the role of epigenetic modifications in evolutionary processes. By leveraging our advanced sequencing technologies for RBP Footprinting and accessibility profiling, we aim to understand how RNA and DNA modifications contribute to adaptation and evolutionary fitness.
Research Experience
Optimizing Bisulfite Sequencing for 5-Methylcytosine Detection in DNA and RNA
Developing Transcriptome-wide Pseudouridine Profiling Method at Base Resolution
Developing Single Base-Resolution Sequencing Methods for m6A
Developing New Cell Lineage Tracing Strategy
Combining Lineage Tracing with Molecular Profiling
Assessing the Influence of Dietary History on Gut Microbiota